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Key Features
Taq DNA Polymerase 2x Master Mix RED is a ready-to-use 2x reaction mix with the Ampliqon Taq DNA polymerase, the NH4+ buffer system, dNTPs and magnesium chloride present. Each reaction requires 25 µl of the 2x Master Mix RED. Simply add primers, template and water to a total reaction volume of 50 µl to successfully carry out primer extensions and other molecular biology applications. Taq DNA Polymerase 2x Master Mix RED offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time. There is no need to buy and use separate loading dyes. Simply load a portion of the reaction product onto an agarose gel for electrophoresis and subsequent visualization. The red dye front runs at 1000 – 2000 bp on a 0.5 – 1.5 % agarose gel
Composition of the Taq DNA Polymerase 2x Master Mix RED (1.5 mM MgCl2 final concentration)
Tris-HCl pH 8.5, (NH4 )2S04 , 3 mM MgCl2 , 0.2% Tween 20
mM of each dNTP
Ampliqon Taq DNA polymerase
Inert red dye and stabilizer
Recommended Storage and Stability
Long term storage at -20 °C. Product expiry at -20 °C is stated on the label
Option: Store at +4 °C for up to 6 months
Quality Control
Taq DNA Polymerase is tested for contaminating activities, with no traces of endonuclease activity, nicking activity or exonuclease activity
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