مستر میکس ریل تایم امپلیکون، RealQ Plus 2x Master Mix Green without ROX کد A323402

Ampliqon, RealQ Plus 2x Master Mix Green without ROX, A323402

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  • مستر میکس ریل تایم امپلیکون، RealQ Plus 2x Master Mix Green without ROX کد A323402: حجم: 1.25 میلی لیتر شرایط نگهداری: 20- درجه سانتی گراد کمپانی: Ampliqon کاتالوگ نامبر: A323402
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Introduction

 The RealQ Plus 2x Master Mix Green without ROX™ is a singletube 2x reagent including all components necessary to perform real-time DNA amplification for DNA-binding dye based PCR. Just add your primers and DNA. No ROX™ internal reference dye level is included 

Detection limit of RealQ Plus Green without ROX™ is approximately 1 copy. Quantification limit is approximately 24 copies (0.08 ng of human gDNA, correlating to 12 diploid genomes, with 2 gene copy per diploid genome) 

Real-time PCR is an important tool for SNP and gene expression analysis

Composition of RealQ Plus 2x Master Mix Green, without ROX

TEMPase Hot Start DNA Polymerase

Optimized buffer system including dNTPs and fluorescent dye

 Recommended Storage and stability

 Long term storage at -20 °C. Product expiry at -20 °C is stated on the label

 Option: Store at +4 °C for up to 3 months

 Quality Control

 TEMPase Hot Start DNA Polymerase is tested for contaminating activities, with no traces of endonuclease activity, nicking activity or exonuclease activity. The RealQ Plus 2x Master Mix Green without ROX™ is functionally tested for efficiency and absence of contaminating human genomic DNA

 Pre-protocol Considerations PCR Primers

It is important - especially in fluorescent DNA dye based quantitative PCR applications - to minimize the formation of non-specific amplification products. Particularly at low target concentration it is important to use the lowest possible primer concentration without compromising the efficiency of the PCR. The optimal concentration of primer pairs is the lowest concentration that results in the lowest Cq and an adequate fluorescence for a given target concentration with minimal or no formation of primer-dimers. The optimal concentrations of upstream and downstream primers are not always of equal molarity. Optimal concentrations of primers are in the range of 100 nM to 800 nM

 Preventing Template Cross-Contamination

 Due to the high sensitivity of quantitative PCR there is a risk of contaminating the reactions with the products of previous runs. To minimize this risk, tubes or plates containing reaction products should not be opened or analysed by gel electrophoresis in the same laboratory area used to set up reactions

Instrument compatibility

Real-time instruments which does not require ROX™ internal reference dye as for example: Bio-Rad CFX96 Touch™, CFX384 Touch™, CFX Connect™, DNA Engine Opticon® 2, Chromo4™, iCycler iQ™ and My iQ™, Roche LightCycler® 480, LightCycler® 1536, LightCycler® Nano, LightCycler® 96 and QuantStudio™ instruments, Thermo Scientific™ PikoReal™, Cepheid SmartCycler®, Bio Molecular Systems Mic qPCR cycler, Qiagen Rotor Gene Q, Rotor Gene 6000, MyGo Mini and MyGo Pro.

مستر میکس ریل تایم امپلیکون، RealQ Plus 2x Master Mix Green without ROX کد A323402
حجم: 1.25 میلی لیتر
شرایط نگهداری: 20- درجه سانتی گراد
کمپانی: Ampliqon
کاتالوگ نامبر: A323402

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