مستر میکس ریل تایم امپلیکون، RealQ Plus 2x Master Mix Green high ROX کد A325402

Ampliqon, RealQ Plus 2x Master Mix Green high ROX, A325402

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  • مستر میکس ریل تایم امپلیکون، RealQ Plus 2x Master Mix Green high ROX کد A325402: حجم: 1.25 میلی لیتر شرایط نگهداری: 20- درجه سانتی گراد کمپانی: Ampliqon کاتالوگ نامبر: A325402
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Introduction

The RealQ Plus 2x Master Mix Green with high ROX™ is a singletube 2x reagent including all components necessary to perform real-time DNA amplification for DNA-binding dye based PCR. Just add your primers and DNA. The ROX™ internal reference dye level is optimized for real-time instruments that require high ROX™ as internal reference dye. See instrument capability

Detection limit of RealQ Plus Green with high ROX™ is approximately 1 copy. Quantification limit is approximately 24 copies (0.08 ng of human gDNA, correlating to 12 diploid genomes, with 2 gene copy per diploid genome)

. Real-time PCR is an important tool for SNP and gene expression analysis

Composition of RealQ Plus 2x Master Mix Green, High ROX

  TEMPase Hot Start DNA Polymerase

 Optimized buffer system including dNTPs, fluorescent dye and ROX™ reference dye

 Recommended Storage and stability 

Long term storage at -20 °C. Product expiry at -20 °C is stated on the label

Option: Store at +4 °C for up to 3 months

Quality Control

TEMPase Hot Start DNA Polymerase is tested for contaminating activities, with no traces of endonuclease activity, nicking activity or exonuclease activity. The RealQ Plus 2x Master Mix Green with high ROX™ is functionally tested for efficiency and absence of contaminating human genomic DNA

 Pre-protocol Considerations

 ROX™ Reference Dye

 ROX™ is used as passive reference dye to compensate for nonPCR related variations in the fluorescence. The ROX™ fluorescence does not change during the course of the PCR reaction nor does it influence the PCR reaction. It provides a stable baseline to which samples are normalized

PCR Primers

 It is important - especially in fluorescent DNA dye based quantitative PCR applications - to minimize the formation of non-specific amplification products. Particularly at low target concentration it is important to use the lowest possible primer concentration without compromising the efficiency of the PCR. The optimal concentration of primer pairs is the lowest concentration that results in the lowest Cq and an adequate fluorescence for a given target concentration with minimal or no formation of primer-dimers. The optimal concentrations of upstream and downstream primers are not always of equal molarity. Optimal concentrations of primers are in the range of 100 nM to 800 nM

Preventing Template Cross-Contamination

Due to the high sensitivity of quantitative PCR there is a risk of contaminating the reactions with the products of previous runs. To minimize this risk, tubes or plates containing reaction products should not be opened or analysed by gel electrophoresis in the same laboratory area used to set up reactions

Instrument compatibility

Real-time instruments which require high ROX™ such as: Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900 HT, StepOne™ and StepOnePlus

مستر میکس ریل تایم امپلیکون، RealQ Plus 2x Master Mix Green high ROX کد A325402
حجم: 1.25 میلی لیتر
شرایط نگهداری: 20- درجه سانتی گراد
کمپانی: Ampliqon
کاتالوگ نامبر: A325402

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