محیط کشت (Dulbecco’s Modified Eagle Medium) DMEM یک محیط پایه است که به طور گسترده ای برای کشت انواع سلول های پستانداران استفاده قرار می گیرد. سلول هایی که می توانند به راحتی و با موفقیت در DMEM کشت داده شوند عبارتند: از فیبروبلاست های اولیه، سلول های عصبی، سلول های عضلانی صاف، رده های سلولی مانند HeLa، 293 ،Cos-7 و PC-12. محیط کشت DMEM با فرمولاسیون های مختلفی تولید میشود که فرمول اصلی و اوریجینال این محیط حاوی 4.5 گرم گلوکز در هر لیتر است. DMEM با گلوکز بالا یا DMEM High Glucose دارای 4.5 گرم در لیتر گلوکز است که برای سلول های با سرعت رشد بالا، سلول هایی که میزان چسبندگی کمی دارند، سلول های سرطانی میلوما و بسیاری از سلول های پرایمری محیط مناسبی است. همچنین بعد از انجام ترنسفکت سلولی و کلونینگ، بهتر است برای رشد و بازیابی سلول از محیط کشت با گلوکز بالا استفاده شود.
محصولات مرتبط
DMEM high glucose powder 10L gibco, 12800082
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool
This DMEM is modified as follows
With | Without |
High Glucose | HEPES |
L-glutamine | Sodium Bicarbonate |
Phenol Red | |
Sodium Pyruvate |
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation
cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM product made in our Scotland facility (12800-116). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard
https://www.thermofisher.com/order/catalog/product/12800082?SID=srch-hj-12800082
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