محیط کشت MEM Alpha (حداقل محیط ضروری) به طور گسترده برای کشت سلولی پستانداران و همچنین انتخاب سلول های DHFR منفی ترانسفکت شده استفاده می شود. MEM α را می توان با انواع سوسپانسیون و سلول های چسبنده پستانداران، از جمله کراتینوسیت ها، آستروسیت های اولیه موش صحرایی و سلول های ملانوم انسانی استفاده کرد.
محصولات مرتبط
Product Overview
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications
:This MEM α is modified as follows
Without | With |
Sodium Bicarbonate | Ribonucleosides |
Deoxyribonucleosides | |
L-glutamine | |
Phenol Red |
Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts
cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco™ MEM α at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation
https://www.thermofisher.com/order/catalog/product/11900073?SID=srch-hj-11900073
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