DMEM/F-12 یک محیط پایه پرکاربرد برای کشت بسیاری از رده های سلولی پستانداران است. سلول های MDCK، سلول های گلیال، فیبروبلاست ها، سلول های اندوتلیال انسان، و همچنین فیبروبلاست های موش صحرایی با موفقیت در محیط کشت DMEM/F12 کشت شدند.
محصولات مرتبط
Product Overview
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications
:This DMEM / F-12 is modified as follows
With | Without |
L-glutamine | HEPES |
Phenol Red | Sodium Bicarbonate |
The complete formulation is available.Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components
cGMP Manufacturing and Quality System
Gibco™ DMEM / F-12 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ DMEM / F-12 product made in our Grand Island facility (12500-096). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation
https://www.thermofisher.com/order/catalog/product/32500035?SID=srch-hj-32500035
دیدگاه خود را بنویسید